DNA Extraction and Shotgun Metagenomics Sequencing of Fecal Samples

This is a Notice of Intent, not a request for proposal. The National Institute of Health intends to negotiate on an other than full and open competition basis with the following vendor: Rush University Medical Center. Acquisition Description: This acquisition requested is for DNA extraction and shotgun metagenomics sequencing of fecal samples from humans (n=25) at baseline and after a ketogenic diet with/with NR supplementation (n=100 samples), and from mice (n=25) at baseline and after a high fat diet with/with NR supplementation (n=100 samples; total 200 samples). The results from these tests will be used to understand cross-species validation of mechanisms related to bile acid- and FXR-induced increases in ketogenesis. Services are required of (1) conducting shotgun metagenomics sequencing of fecal samples for 200 samples (including DNA extraction, library preparation and shotgun metagenomics sequencing) and (2) providing raw data files to the PI, Dr. Katherine Maki. The statutory authority for this sole source requirement is 41 U.S.C. 253(c) (1) as implemented by FAR 6.302-1 only one responsible source and no other supply of service will satisfy agency requirements. THIS IS NOT A REQUEST FOR PROPOSAL. All responsible sources that could provide the required may submit a capability statement that will be considered by email (subject line to reference NOI-CC-OLAO-26-005551) by 12:00 PM eastern standard time on 05/23/2026 to: valerie.gregorio@nih.gov. All responses received by the closing date of this synopsis will be considered by the Government. A determination not to compete this requirement, based upon responses to this notice, is solely within the discretion of the Government.